Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

A New Approach to Monitoring and Evaluation of Cecal Ligation and Puncture Sepsis Model

Background: Sepsis is a systemic inflammatory disease in response to the pathogens that leads to vital organ failures the failure of vital organs. Appropriate animal models should be developed to measure the effectiveness of therapeutic methods. Cecal Ligation and Puncture (CLP) is the most widely used methods of creating the sepsis model. Some variables interfere in the creation of the CLP mod...

Cecal ligation and puncture induced sepsis

Purpose: Multiresistant and vancomycin resistant Enterococcus faecium (VRE) can cause serious infections in hospitalized patients with various co-morbid diseases. We investigated the course of VRE peritonitis after cecal ligation and puncture (CLP)-induced sepsis and compared this to sham operated mice. Methods: Mice were subjected to CLP or sham surgery. Forty-eight hours thereafter four group...

Protective role of autophagy in mouse cecal ligation and puncture-induced sepsis model

Kinetics and protective role of autophagy in a mouse cecal ligation and puncture-induced sepsis

INTRODUCTION It is not well understood whether the process of autophagy is accelerated or blocked in sepsis, and whether it is beneficial or harmful to the immune defense mechanism over a time course during sepsis. Our aim was to determine both the kinetics and the role of autophagy in sepsis. METHODS We examined autophagosome and autolysosome formation in a cecal ligation and puncture (CLP) ...

Sepsis modeling in mice: ligation length is a major severity factor in cecal ligation and puncture

BACKGROUND The cecal ligation and puncture (CLP) model, a gold standard in sepsis research, is associated with an important variability in mortality. While the number of punctures and needle size is well described in CLP animal studies, the length of cecal ligation is often not. The relationship between cecal ligation and survival in mice is briefly reported in the literature; therefore, we dev...